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油菜转基因育种研究进展

官春云

《中国工程科学》 2002年第4卷第8期页码 34-39

摘要：

简要介绍了国外油菜转基因育种情况，包括转除草剂抗性基因，病原菌抗性基因，耐重金属基因，影响种子贮藏产物基因，影响繁殖特性基因，医药和工业产品基因，以及启动子调节基因等。较详细介绍了湖南农业大学在Bt毒蛋白基因转化甘蓝型油菜育成抗虫新品系、基因工程雄性不育系、恢复系选育和杂种优势利用，以及反义FAD2基因转化甘蓝型油菜双低品种提高油酸含量的研究结果；浙江省农科院利用反义PEP基因转化甘蓝型油菜提高种子含油量的研究结果。

关键词：油菜转基因育种

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Mercury enrichment in

Chunhao Dai, Pufeng Qin, Zhangwei Wang, Jian Chen, Xianshan Zhang, Si Luo

《环境科学与工程前沿（英文）》 2017年第11卷第1期 doi: 10.1007/s11783-017-0892-z

摘要： Mercury enrichment in response to elevated atmospheric mercury concentrations in the organs of rape ( ) was investigated. Elevated soil mercury content had significant impact on mercury accumulation in rape stems, roots, seeds and seed coats. Leaf mercury in the leaves was mostly affected by atmospheric input while the stems were mostly affected by soil concentrations. Mercury in the aboveground plant tissue mainly derived from atmospheric absorption, and atmospheric mercury absorption in leaves was higher than that in the stems. Mercury enrichment in response to elevated atmospheric mercury concentrations in the organs of rape ( ) was investigated using an open top chamber fumigation experiment and a soil mercury enriched cultivation experiment. Results indicate that the mercury concentration in leaves and stems showed a significant variation under different concentrations of mercury in atmospheric and soil experiments while the concentration of mercury in roots, seeds and seed coats showed no significant variation under different atmospheric mercury concentrations. Using the function relation established by the experiment, results for atmospheric mercury sources in rape field biomass showed that atmospheric sources accounted for at least 81.81% of mercury in rape leaves and 32.29% of mercury in the stems. Therefore, mercury in the aboveground biomass predominantly derives from the absorption of atmospheric mercury.

关键词： Open top chamber Gaseous elemental mercury (GEM) Soil Mercury Brassica napus

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高油酸油菜中甘油三酯的组成及结构研究 Artical

官梅,陈洪,熊兴华,吕昕,李栒,黄凤洪,官春云

《工程（英文）》 2016年第2卷第2期页码 258-262 doi: 10.1016/J.ENG.2016.02.004

摘要：

植物油中甘油三酯(TAG)的脂肪酸组成及其在甘油主链上的位置决定了植物油脂的营养价值。本研究采用气相色谱法(GC)和高效液相色谱– 串联质谱联用(HPLC-MS/MS)分析了不同油酸含量的甘蓝型油菜籽油的甘油三酯的脂肪酸组成和结构。结果表明：高油酸油菜籽油的油酸含量为80%左右。TAG酰基总碳数(CN值)为54的甘油三酯的相对含量占80%以上。主要的甘油三酯为OOO，相对含量最高达71.75%，其次是OOL 7.56%、OOLn 4.81%和SOO 4.74%。在高油酸的品种中油酸主要集中在甘油三酯上的sn-2位，而在高芥酸品种中，油酸主要集中在甘油三酯上的sn-1/3位置。结果表明，高油酸油菜籽油是一种具有极高营养价值的植物油。

关键词：油菜脂肪酸甘油三酯位置异构体

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Identification of pakchoi cultivars with low cadmium accumulation and soil factors that affect their cadmium uptake and translocation

Lin WANG,Yingming XU,Yuebing SUN,Xuefeng LIANG,Dasong LIN

《环境科学与工程前沿（英文）》 2014年第8卷第6期页码 877-887 doi: 10.1007/s11783-014-0676-7

摘要： The selection and use of low-Cd-accumulating cultivar (LCAC) has been proposed as one of the promising approaches in minimizing the entry of Cd in the human food chain. This study suggests a screening criterion of LCACs focusing on food safety. Pot culture and plot experiments were conducted to screen out LCACs from 35 pakchoi cultivars and to identify the crucial soil factors that affect Cd accumulation in LCACs. Results of the pot culture experiment showed that shoot Cd concentrations under the three Cd treatments significantly varied across cultivars. Two cultivars, Hualv 2 and Huajun 2, were identified as LCACs because their shoot Cd concentrations were lower than 0.2 mg·kg under low Cd treatment and high Cd exposure did not affect the biomass of their shoots. The plot experiment further confirmed the consistency and genotypic stability of the low-Cd-accumulating traits of the two LCACs under various soil conditions. Results also showed that soil phosphorus availability was the most important soil factor in the Cd accumulation of pakchoi, which related negatively not only to Cd uptake by root but also to Cd translocation from root to shoot. The total Cd accumulation and translocation rates were lower in the LCACs than in the high-Cd cultivar, suggesting that Cd accumulation in different cultivars is associated with the Cd uptake by root as well as translocation from root to shoot. This study proves the feasibility of the application of the LCAC strategy in pakchoi cultivation to cope with Cd contamination in agricultural soils.

关键词： pakchoi (Brassica chinensis L.) cadmium contamination low-cadmium-accumulating cultivar (LCAC) genotype variation soil factor translocation

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Comparisons in subcellular and biochemical behaviors of cadmium between low-Cd and high-Cd accumulation cultivars of pakchoi (

Meng XUE, Yihui ZHOU, Zhongyi YANG, Biyun LIN, Jiangang YUAN, Shanshan WU

《环境科学与工程前沿（英文）》 2014年第8卷第2期页码 226-238 doi: 10.1007/s11783-013-0582-4

摘要： Subcellular distributions and chemical forms of cadmium (Cd) in the leaves, stems and roots were investigated in low-Cd accumulation cultivars and high-Cd accumulation cultivars of pakchoi ( L.). Root cell wall played a key role in limiting soil Cd from entering the protoplast, especially in the low-Cd cultivars. The high-Cd cultivars had significantly higher leaf and stem Cd concentrations than the low-Cd cultivars in cell wall fraction, chloroplast/trophoplast fraction, organelle fraction and soluble fraction. In low-Cd cultivars, which were more sensitive and thus had greater physiological needs of Cd detoxification than high-Cd cultivars, leaf vacuole sequestrated higher proportions of Cd. Cd in the form of pectate/protein complexes (extracted by 1 mol·L NaCl) played a decisive role in Cd translocation from root to shoot, which might be one of the mechanisms that led to the differences in shoot Cd accumulation between the two types of cultivars. Furthermore, the formation of Cd-phosphate complexes (extracted by 2% HAc) was also involved in Cd detoxification within the roots of pakchoi under high Cd stress, suggesting that the mechanisms of Cd detoxification might be different between low- and high-Cd cultivars.

关键词： cadmium (Cd) low-Cd cultivar pakchoi (Brassica chinensis L.) subcellular distribution chemical forms